---
title: Pilot Study of Protein Profiling of the Human Epidermal Cells After Ultraviolet Irradiation
nct_id: NCT00397436
overall_status: COMPLETED
phase: EARLY_PHASE1
sponsor: University of Minnesota
study_type: INTERVENTIONAL
primary_condition: Proteomics
countries: United States
canonical_url: "https://parkinsonspathways.com/agent/trials/NCT00397436.md"
clinicaltrials_gov: "https://clinicaltrials.gov/study/NCT00397436"
ct_last_update_post_date: 2016-05-17
last_seen_at: "2026-05-12T07:20:53.985Z"
source: ClinicalTrials.gov (mirrored, no enrichment)
---
# Pilot Study of Protein Profiling of the Human Epidermal Cells After Ultraviolet Irradiation

**NCT ID:** [NCT00397436](https://clinicaltrials.gov/study/NCT00397436)

## Key Facts

- **Status:** COMPLETED
- **Phase:** EARLY_PHASE1
- **Study Type:** INTERVENTIONAL
- **Target Enrollment:** 60
- **Lead Sponsor:** University of Minnesota
- **Conditions:** Proteomics
- **Start Date:** 2007-02
- **Completion Date:** 2015-12
- **CT.gov Last Update:** 2016-05-17

## Brief Summary

The main focus of this pilot study is to construct a fairly complete protein profile of the epidermis. This will allow us to study how the epidermis responds to external stressors such as ultra-violet. We hypothesize that by using the cutting edge technology, proteomics, we will be able to construct a fairly complete profile of different epidermal cells (keratinocytes, epidermal nerve fibers (ENFs), melanocytes, Langerhans cell,...etc) of normal and ultraviolet (UV) irradiated skin.

## Detailed Description

We will investigate the changes of the epidermal cell proteome after UV irradiation to the forearms of healthy young male volunteers. Knowledge of specific molecular markers may provide knowledge of basic biological systems and possibly targeted therapy of inflammation.

By using combined proteomic methodologies we will be able to quantify protein changes at cytoplasmic and membrane protein levels of epidermal cells in normal vs. irradiated skins.

This study may allow us to obtain fairly comprehensive profile of membrane integrated and cytoplasmic proteins of epidermal cell populations which will facilitate our understand of biological responses to UV irradiation.

## Eligibility

- **Minimum age:** 18 Years
- **Maximum age:** 30 Years
- **Sex:** MALE
- **Healthy Volunteers:** Yes

```
Inclusion Criteria:

* Healthy Males age 18-30
* Caucasian
* No neurological disease

Exclusion Criteria:

* Use of any medication that affects skin
```

## Arms

- **1** (EXPERIMENTAL) — Comparing irradiated skin to non irradiated skin.

## Interventions

- **UV Irradiation** (PROCEDURE) — UV irradiation

## Primary Outcomes

- **Ability to quantify protein changes at cytoplasmic and membrane protein levels of epidermal cells in normal vs. irradiated skin** _(time frame: 1 year)_

## Secondary Outcomes

- **Obtain a profile of membrane integrated and cytoplasmic proteins of epidermal cell populations which will facilitate our understanding of biological responses to UV irradiation.** _(time frame: 1 year)_

## Locations (1)

- University of Minnesota, Minneapolis, Minnesota, United States

## Recent Field Changes (last 30 days)

- `status.overallStatus` — added _(2026-05-12)_
- `status.primaryCompletionDate` — added _(2026-05-12)_
- `status.completionDate` — added _(2026-05-12)_
- `status.lastUpdatePostDate` — added _(2026-05-12)_
- `design.phases` — added _(2026-05-12)_
- `design.enrollmentCount` — added _(2026-05-12)_
- `eligibility.criteria` — added _(2026-05-12)_
- `eligibility.minAge` — added _(2026-05-12)_
- `eligibility.maxAge` — added _(2026-05-12)_
- `eligibility.sex` — added _(2026-05-12)_
- `outcomes.primary` — added _(2026-05-12)_
- `outcomes.secondary` — added _(2026-05-12)_
- `armsInterventions.arms` — added _(2026-05-12)_
- `armsInterventions.interventions` — added _(2026-05-12)_
- `sponsor.lead` — added _(2026-05-12)_
- `results.hasResults` — added _(2026-05-12)_
- `locations.university of minnesota|minneapolis|minnesota|united states` — added _(2026-05-12)_

---

*Canonical: https://parkinsonspathways.com/agent/trials/NCT00397436.md*  
*Source data (authoritative): https://clinicaltrials.gov/study/NCT00397436*  
*This page is a raw mirror with no AI summary, no editorial enrichment, and no Parkinson's-specific filtering.*