---
title: Effects of Isotonic Beetroot Supplementation on Sprint Interval Exercise Performance
nct_id: NCT06349018
overall_status: COMPLETED
phase: NA
sponsor: Nanyang Technological University
study_type: INTERVENTIONAL
primary_condition: Exercise Performance
countries: Singapore
canonical_url: "https://parkinsonspathways.com/agent/trials/NCT06349018.md"
clinicaltrials_gov: "https://clinicaltrials.gov/study/NCT06349018"
ct_last_update_post_date: 2025-04-13
last_seen_at: "2026-05-12T06:05:28.485Z"
source: ClinicalTrials.gov (mirrored, no enrichment)
---
# Effects of Isotonic Beetroot Supplementation on Sprint Interval Exercise Performance

**Official Title:** The Effects of Isotonic Beetroot Supplementation on Sprint Interval Exercise Performance.

**NCT ID:** [NCT06349018](https://clinicaltrials.gov/study/NCT06349018)

## Key Facts

- **Status:** COMPLETED
- **Phase:** NA
- **Study Type:** INTERVENTIONAL
- **Target Enrollment:** 24
- **Lead Sponsor:** Nanyang Technological University
- **Conditions:** Exercise Performance
- **Start Date:** 2024-02-20
- **Completion Date:** 2024-05-31
- **CT.gov Last Update:** 2025-04-13

## Brief Summary

This study aims to examine the effects of dietary nitrate supplementation via beetroot juice on sprint interval cycling exercise performance. Twenty-four participants (male or female) will undertake a randomized, crossover trial where they consume either (i) an isotonic beetroot juice drink containing nitrate (12.9 mmol) for 5 days or (ii) an isotonic sports drink (no nitrate) for 5 days. On Day 5 of each trial, participants will come to the laboratory and 2 hours after consuming their allocated drink will complete a 6 bouts of sprint interval exercise. Power output during cycling will be measured as the primary outcome measure. Salivary and plasma measures of nitrate and nitrite will be taken over the course of the morning on Day 5. After a 5-day washout period participants will crossover and begin their second trial.

## Detailed Description

This study aims to examine the effects of dietary nitrate supplementation via beetroot juice on sprint interval cycling exercise performance. Twenty-four participants (male or female) will undertake a randomized, crossover trial where they consume either (i) an isotonic beetroot juice drink containing nitrate (12.9 mmol) for 5 days or (ii) an isotonic sports drink (no nitrate) for 5 days. Drinks will be equivalent in isotonicity and colored the same so that participants and investigators will be blinded to each trial. Randomization and drink allocation will be completed by a third person not associated with the study team. On Day 5 of each trial, participants will come to the laboratory and 2 hours after consuming their allocated drink will complete a 6 bouts of sprint interval exercise (10 second all-out maximal sprints interspersed with 50 seconds rest). Power output (peak and mean) during cycling sprints will be measured as the primary outcome measure. Salivary measures of nitrate/nitrite will be taken at three time points over the course of the morning on Day 5 - (i) pre-drink consumption; (ii) 2 hours post-drink consumption; and (iii) post-exercise. Plasma measures of nitrate and nitrite will be taken at two time points over the course of the morning on Day 5 - (i) pre-drink consumption; and (ii) 2 hours post-drink consumption. Near infra-red spectroscopy (NIRS) will be used to measure muscle (vastus lateralis) oxygenation during cycling bouts. A Stroop test to measure inhibition (executive function) will be taken pre- and post-exercise. Sensory perception of the drink will be measured immediately post-consumption on Day 5. After a 5-day washout period participants will crossover and begin their second trial.

## Eligibility

- **Minimum age:** 21 Years
- **Maximum age:** 40 Years
- **Sex:** ALL
- **Healthy Volunteers:** Yes

```
Inclusion Criteria:

* Age: 21 - 40 years
* Healthy and injury-free (e.g., no cardiovascular disease, diabetes mellitus, orthopaedic impairment that interferes with moderate-to-vigorous exercise)
* Blood pressure \< 130/90 mmHg
* Be physically active: Engaging in a minimum of 150 minutes of moderate-intensity exercise or 75 minutes of vigorous-intensity physical activity each week.
* Recreational cyclist (Minimum 1 hour cycling per week).
* Body mass index: 18.5-25 kg/m2
* Non-smoker
* Asian

Exclusion Criteria:

* Any allergies to beetroot or isotonic drink.
* Asthma
* Any diagnosed form of cardiometabolic disease (heart disease, stroke, peripheral vascular disease, diabetes, metabolic syndrome, high blood pressure).
* Any symptoms contraindicating exercise testing (e.g. chest pains).
* Any balance or dizziness problems.
* Any chronic medical conditions (whether medicated or not).
* Any bone joint problems.
* Any Physician diagnosed contraindications to exercise.
* Any other health problems that may be a cause for concern or contraindication to exercise.
* Any recent Covid vaccination (within last 2 weeks).
```

## Arms

- **High nitrate** (EXPERIMENTAL) — High nitrate isotonic drink
- **No nitrate** (ACTIVE_COMPARATOR) — No nitrate isotonic drink

## Interventions

- **Dietary Supplement: High nitrate isotonic drink** (OTHER) — High nitrate (12.9 mmol) drink with carbohydrate and minerals
- **Dietary Supplement: No nitrate isotonic drink** (OTHER) — Carbohydrate and mineral isotonic drink

## Primary Outcomes

- **Power output during maximal sprint cycling** _(time frame: 2 hours after drink ingestion)_ — Peak and mean power output

## Secondary Outcomes

- **Salivary nitrate** _(time frame: Pre-drink, 2 hours post-drink and immediately post-exercise (2.5 hours post-drink))_
- **Plasma nitrate and nitrite** _(time frame: Pre-drink and 2 hours post-drink)_
- **Muscle oxygenation** _(time frame: During exercise (2-2.5 hours post drink))_
- **Sensory evaluation (Acceptance and Preference Test Evaluation by Meilgaard et al 2007)** _(time frame: Immediately post-drink (1 minute post-drink))_
- **Stroop test** _(time frame: Immediately pre- (2 hours post-drink) and immediately post-exercise (2.5 hours post drink))_

## Locations (1)

- Human Bioenergetics Laboratory, National Institute of Education, Nanyang Technological University, Singapore, Singapore

## Recent Field Changes (last 30 days)

- `status.overallStatus` — added _(2026-05-12)_
- `status.primaryCompletionDate` — added _(2026-05-12)_
- `status.completionDate` — added _(2026-05-12)_
- `status.lastUpdatePostDate` — added _(2026-05-12)_
- `design.phases` — added _(2026-05-12)_
- `design.enrollmentCount` — added _(2026-05-12)_
- `eligibility.criteria` — added _(2026-05-12)_
- `eligibility.minAge` — added _(2026-05-12)_
- `eligibility.maxAge` — added _(2026-05-12)_
- `eligibility.sex` — added _(2026-05-12)_
- `outcomes.primary` — added _(2026-05-12)_
- `outcomes.secondary` — added _(2026-05-12)_
- `armsInterventions.arms` — added _(2026-05-12)_
- `armsInterventions.interventions` — added _(2026-05-12)_
- `sponsor.lead` — added _(2026-05-12)_
- `results.hasResults` — added _(2026-05-12)_
- `locations.human bioenergetics laboratory, national institute of education, nanyang technological university|singapore||singapore` — added _(2026-05-12)_

---

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*Source data (authoritative): https://clinicaltrials.gov/study/NCT06349018*  
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