--- title: "Clinical and Microbiological Evaluation of Laser Assisted New Attachment Procedure (LANAP) Using Nd:Yag vs. Diode Laser in the Management Of Stage II Periodontitis" nct_id: NCT06358937 overall_status: UNKNOWN phase: NA sponsor: Mahmoud Salem study_type: INTERVENTIONAL primary_condition: Periodontal Diseases countries: Egypt canonical_url: "https://parkinsonspathways.com/agent/trials/NCT06358937.md" clinicaltrials_gov: "https://clinicaltrials.gov/study/NCT06358937" ct_last_update_post_date: 2024-04-11 last_seen_at: "2026-05-12T07:12:23.785Z" source: ClinicalTrials.gov (mirrored, no enrichment) --- # Clinical and Microbiological Evaluation of Laser Assisted New Attachment Procedure (LANAP) Using Nd:Yag vs. Diode Laser in the Management Of Stage II Periodontitis **Official Title:** Clinical and Microbiological Evaluation of Laser Assisted New Attachment Procedure (LANAP) Using Nd:Yag vs. Diode Laser in the Management Of Stage II Periodontitis (Randomized Controlled Clinical Trial) **NCT ID:** [NCT06358937](https://clinicaltrials.gov/study/NCT06358937) ## Key Facts - **Status:** UNKNOWN - **Phase:** NA - **Study Type:** INTERVENTIONAL - **Target Enrollment:** 27 - **Lead Sponsor:** Mahmoud Salem - **Conditions:** Periodontal Diseases - **Start Date:** 2023-11-01 - **Completion Date:** 2024-05 - **CT.gov Last Update:** 2024-04-11 ## Brief Summary The aim of the present study is to compare the efficacy of LANAP to conventional scaling and root planing in the management of stage II periodontitis. ## Eligibility - **Minimum age:** 30 Years - **Maximum age:** 50 Years - **Sex:** ALL - **Healthy Volunteers:** No ``` Inclusion Criteria: * Patients with stage II generalized periodontitis PPD ≥ 5 mm with attachment loss 3-4 mm with horizontal bone loss Exclusion Criteria: * Patients with vertical bone loss or furcation involvement. * History of smoking more than (10 cigarettes / day). * Patient with medical condition that contraindicate surgical procedures. * Patients receiving antibiotics in the past three months prior to the procedure. * Pregnant or lactating females. ``` ## Arms - **Laser Assisted New Attachment Procedure using ND:YAG laser** (EXPERIMENTAL) - **Laser Assisted New Attachment Procedure using diode laser** (EXPERIMENTAL) - **Scaling and Root Planing using ultrasonic and curettes** (ACTIVE_COMPARATOR) ## Interventions - **Laser Assisted New Attachment Procedure using ND:YAG laser** (DEVICE) — A free running, pulsed, 1064 nm wavelength-specific ND:YAG laser will be used. A thin 300 μm laser fiber optic will be placed parallel to the root surface, allows easy access into deep periodontal pockets. The initial pass with the laser is known as Laser Troughing, and it is done with a micro-short duration pulse, with a 4.0 watt power, 150 μs duration in pulsed mode and 20Hz frequency. Ultrasonic scaler will be then used to remove calculus. The second pass is carried out with 650 μs pulse duration, 10-30 seconds for each tooth, 4.0 watt power and 20HZ frequency, to improve the ability to generate a fibrin clot. The fibrin clot is then compressed. Relieving the occlusion is the last step of the LANAP protocol. - **Laser Assisted New Attachment Procedure using diode laser** (DEVICE) — Diode laser (Biolase) with 940 nm wavelength, a thin flexible fiber-optic cable 300 μm attached with a power average set at 0.5-1.5 watt will be used. The first pass "laser troughing" is done using a thin flexible fiber-optic cable 300 μm placed parallel to the root surface, attached with a power average set at 0.5-1 watt, in continuous mode. Ultrasonic scaler will be then used to remove calculus which present on the root surface. The second pass is carried out with 1-1.5 watt power in continuous mode, 10-30 seconds for each tooth, and, to improve the ability to generate a fibrin clot. The fibrin clot is then compressed in order to enhance the healing - **Scaling and Root Planing using ultrasonic and curettes** (PROCEDURE) — Scaling and root planing (SRP) using ultrasonic device at a moderate setting and with the appropriate tips and curettes will be also used where indicated and time spent in SRP on each tooth will not be restricted. ## Primary Outcomes - **Microbiological assessment of Fusobacterium nucleatum** _(time frame: up to 6 months)_ — Subgingival plaque biofilm will be collected using paper cone after making a good isolation of the operated field and put into a sterile microcentrifuge tube containing phosphate buffered saline to be transferred immediately to the Microbiology laboratory for analysis of microbiome. Microcentrifuge tubes will be vortexed for 5 minutes then 200 ul of the resulting suspension will be subjected to DNA extraction using QIAamp DNA minikit. Specific PCR primers targeting gingival plaque associated oral microbiota (Fusobacterium nucleatum) will be used in SYBR Green Real-Time PCR. Amplification of 16SrRNA gene will be used as the denominator against which the amplification of other bacteria will be estimated. The bacterial relative quantification will be calculated automatically by the Rotor-Gene software and expressed as relative fold difference. - **Microbiological assessment of Porphyromonas gingivalis** _(time frame: up to 6 months)_ — Subgingival plaque biofilm will be collected using paper cone after making a good isolation of the operated field and put into a sterile microcentrifuge tube containing phosphate buffered saline to be transferred immediately to the Microbiology laboratory for analysis of microbiome. Microcentrifuge tubes will be vortexed for 5 minutes then 200 ul of the resulting suspension will be subjected to DNA extraction using QIAamp DNA minikit. Specific PCR primers targeting gingival plaque associated oral microbiota (Porphyromonas gingivalis) will be used in SYBR Green Real-Time PCR. Amplification of 16SrRNA gene will be used as the denominator against which the amplification of other bacteria will be estimated. The bacterial relative quantification will be calculated automatically by the Rotor-Gene software and expressed as relative fold difference. - **Microbiological assessment of Tannerella forsythia** _(time frame: up to 6 months)_ — Subgingival plaque biofilm will be collected using paper cone after making a good isolation of the operated field and put into a sterile microcentrifuge tube containing phosphate buffered saline to be transferred immediately to the Microbiology laboratory for analysis of microbiome. Microcentrifuge tubes will be vortexed for 5 minutes then 200 ul of the resulting suspension will be subjected to DNA extraction using QIAamp DNA minikit. Specific PCR primers targeting gingival plaque associated oral microbiota (Tannerella forsythia) will be used in SYBR Green Real-Time PCR. Amplification of 16SrRNA gene will be used as the denominator against which the amplification of other bacteria will be estimated. The bacterial relative quantification will be calculated automatically by the Rotor-Gene software and expressed as relative fold difference. ## Secondary Outcomes - **Gingival index** _(time frame: up to 6 months)_ - **Plaque index** _(time frame: up to 6 months)_ - **Clinical attachment loss** _(time frame: up to 6 months)_ - **Probing depth** _(time frame: up to 6 months)_ ## Locations (1) - Faculty of Dentistry, Alexandria University, Alexandria, Egypt — _RECRUITING_ ## Recent Field Changes (last 30 days) - `eligibility.minAge` — added _(2026-05-12)_ - `eligibility.maxAge` — added _(2026-05-12)_ - `eligibility.sex` — added _(2026-05-12)_ - `outcomes.primary` — added _(2026-05-12)_ - `status.overallStatus` — added _(2026-05-12)_ - `status.primaryCompletionDate` — added _(2026-05-12)_ - `status.completionDate` — added _(2026-05-12)_ - `status.lastUpdatePostDate` — added _(2026-05-12)_ - `design.phases` — added _(2026-05-12)_ - `design.enrollmentCount` — added _(2026-05-12)_ - `eligibility.criteria` — added _(2026-05-12)_ - `outcomes.secondary` — added _(2026-05-12)_ - `armsInterventions.arms` — added _(2026-05-12)_ - `armsInterventions.interventions` — added _(2026-05-12)_ - `sponsor.lead` — added _(2026-05-12)_ - `results.hasResults` — added _(2026-05-12)_ - `locations.faculty of dentistry, alexandria university|alexandria||egypt` — added _(2026-05-12)_ --- *Canonical: https://parkinsonspathways.com/agent/trials/NCT06358937.md* *Source data (authoritative): https://clinicaltrials.gov/study/NCT06358937* *This page is a raw mirror with no AI summary, no editorial enrichment, and no Parkinson's-specific filtering.*